|Place of Birth||Azerbaijan, Divichi district|
|Date of Birth||02.05.1958|
|Education||Azerbaijan State University|
|Scientific degree||Doctor of Biological Sciences|
|Topic of PhD thesis:
- specialty code
- specialty name- topic name
BiochemistryPurification and physicochemical properties of carbonic anhydrase from chickpea leaves
|Topic of doctoral thesis:
- specialty code
- specialty name- topic name
Molecular biologyCharacterization and expression analysis of PGPase and PEP-carboxylase genes and their catalytic subunits from unicellular green algae Chlamydomonas reinhardtii
|Election of corresponding member of ANAS:
- date- specialty name
|Total number of printed scientific publications:
- number of scientific publications printed abroad:- number of papers published in journals indexed and abstracted in international databases
|Certificates of authorship and number of patents||16|
- number of PhD
- number of Doctor of sciences
|Main scientific achievements||Developed a strategy of enzymatic deglycosylation of target proteins in vivo by co-introducing bacterial PNGase F, which allows for producing functional active valuable recombinant proteins, vaccines, therapeutic proteins, antibodies etc. in eukaryotic system including plants in a non-N-glycosylated form.
Discovered that Chlamydomonas reinhardtii green algae possess glycoproteins with mammalian-like sialylated N-linked oligosaccharides.
Discovered novel Phosphoenolpyruvate Carboxylase genes and their active polypeptides in the green microalga Chlamydomonas reinhardtii, which are essential for high rates of carbon dioxide fixation in plants/algae, i.e., for biomass accumulation and biofuel production.
Developed the efficient procedure for the purification of PGPase in a green alga C. reinhardtii that enabled to get the world’s first purified enzyme in eukaryotes, which allowed determining the sequence of PGPase from many organisms. This finding was the first molecular and enzyme characterization of PGPase in any eukaryotic organisms including human.
Discovered the importance of the annealing time for successful amplification of high GC DNA regions.
Developed a rational platform for gene synthesis using a fast polymerase chain reaction that provided the world's fastest synthetic gene synthesis.
The carbonic anhydrase was purified, characterized and crystallized from Cicer arietinum leaves for the first time and molecular model of its quaternary structure was proposed.
|Names of scientific works||1. Production of Functionally Active and Immunogenic Non-Glycosylated Protective Antigen from Bacillus anthracis in Nicotiana benthamiana by Co-Expression with Peptide-N-Glycosidase F (PNGase F) of Flavobacterium meningosepticum. PLoS One. 2016, 11(4).
2. Development of a Single-Replicon miniBYV Vector for Co-expression of Heterologous Proteins. Mol Biotechnol. 2015, 57(2), 101-10.
3. In vivo deglycosylation of recombinant proteins in plants by co-expression with bacterial PNGase F. Bioengineered. 2013, 17, 4(5).
4. Production of non-glycosylated recombinan proteins in Nicotiana benthamiana plants by co-expressing bacterial PNGase F. Plant Biotechnology Journal, 2012, 10(7), 773-82.
5. Co-Expression of Bacterial Enzyme PNGase F In Vivo Allows for Producing Recombinant Proteins of Interest in Plants in a Non-N-Glycosylated Form. ISB News Report, 2013, Jan., 5-8.
6. Antibodies to plant-produced Plasmodium falciparum sexual stage protein Pfs25 exhibit transmission blocking activity. Human vaccine, 2011, 7, 191–198.
7. Green algae Chlamydomonas reinhardtii possess endogenous sialylated N-glycans. FEBS Open Bio, 2011, 1, 15-22.
8. The effect of ultrasound stimulation on the gene and protein expression of chondrocytes seeded in chitosan scaffolds. J Tissue Eng Regen Med. 2011, 5(10), 815-822.
9. Sequential co-immobilization of thrombomodulin and endothelial protein C receptor on polyurethane: activation of protein C.Acta Biomater. 2011 Jun;7(6):2508-17.
10. Automated two-column purification of Iminobiotin and BrdU labeled PCR products for rapid cloning: application to genes synthesized by Polymerase Chain Assembly. J Chromatogr Sci. 2010, 48(2):120-124
11. Gene synthesis by integrated polymerase chain assembly and PCR amplification using a high-speed thermocycler. J Microbiol Methods. 2009, 79(3):295-300.
12. Gene synthesis by integrated polymerase chain assembly and PCR amplification using a high-speed thermocycler. J Microbiol Methods. 2009, 79(3):295-300.
13. Molecular chaperon activity of tomato (Lycopersicon esculentum Mill.) Endoplasmic Reticulum-Located Small Heat-Shock Protein. Journal of Plant Research, 2008, 121: 235-243.
14. A Fundamental Study of the PCR Amplification of GC-Rich DNA Templates. Comput. Biol. Chem., 2008, 32(6): 452-457.
15. Rational de novo gene synthesis by rapid polymerase chain assembly (PCA) and expression of endothelial protein-C and thrombin receptor genes. J Biotechnology, 2007, 131(4): 379-387.
16. The two divergent PEP-carboxylase catalytic subunits in the green microalga Chlamydomonas reinhardtii respond reversibly to inorganic-N supply and co-exist in the high-molecular-mass, hetero-oligomeric Class-2 PEPC complex. FEBS Letters, 581: 4871-4876.
17. Identification and expression analysis of two inorganic C-and N responsive genes encoding novel and distinct molecular forms of eukaryotic phosphoenolpyruvate carboxylase in the green microalga Chlamydomonas reinhardtii. The Plant Journal, 2005, 42: 832–843.
18. Phosphoglycolate phosphatase gene and the mutation in phosphoglycolate phosphatase-deficient mutant (pgp1) of Chlamydomonas reinhardtii. Can. J. Botany, 83: 842-849.
19. Characteristics and Sequence of Phosphoglycolate Phosphatase from an Eukaryotic Green Alga Chlamydomonas reinhardtii. J Biol. Chem., 2001, 276 (49): 45573-45579.
20. A mutant of Chlamydomonas reinhardtii with Reduced Rate of Photorespiration. Plant Cell Physiol., 40(8): 792-799.
21. Physicochemical properties and quaternary structure of carbonic anhydrase from Cicer arietinum leaves. Biochemistry, 51: 1785-1794.
22. Quaternary structure of carbonic anhydrase from leaves of dicotyledonous plant Cicer arietinum. Doklady Academii Nauk, USSR Academy of Sciences 285(6): 1472-1475.
23. Subunit structure of carbonic anhydrase from leaves of chickpea. Doklady Academii Nauk, USSR Academy of Sciences, 280(6): 1466-1468.
|Membership with international and foreign scientific organizations||Genetics Society of America
The Japanese Society of Plant Physiologists
American Society of Plant Biologists
Turkish Biochemical Society
|Pedagogical activity||2002-2009 Nebraska- Lincoln University, USA Professor
2014- Akdeniz University, Tukkey, Antalya, Professor
|Other activities||Editorial Board Member:
International Journal of Plant Genomics
Journal of Plant Studies
|Awarding and prizes||- Award for The STA (Japan Science and Technology Agency) Fellowship, 1998
- Award for The STA Fellowship, 1999-2001
- Award for the JIRCAS Visiting Fellowship, 2001-2002
|Place of work and its address||Akdeniz University, Department of Agrobiotechnological Biotechnology, Dumlupınar Boulevard, 07058 Campus; Antalya, Turkey|
|Office phone||(+90 242) 2274700|
|Mobil||(+90 537) 2024759|
|Home phone||(+90 535) 3167515|
|Fax||(+90 242) 2275540|